2V4V
Crystal Structure of a Family 6 Carbohydrate-Binding Module from Clostridium cellulolyticum in complex with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113.15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.026, 43.598, 93.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.250 - 1.500 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gmm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.587 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.250 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 20980 | |
| <I/σ(I)> | 13.1 | 3.6 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.37 | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.1M NAOAC, 0.1M NACITRATE.2H20 PH 5.8 |
