2V3F
acid-beta-glucosidase produced in carrot
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.088, 97.104, 82.932 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 41.560 - 1.950 |
| R-factor | 0.154 |
| Rwork | 0.149 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.371 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.250 |
| Number of reflections | 74750 | |
| <I/σ(I)> | 13.6 | 6.3 |
| Completeness [%] | 97.0 | 98.9 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | AMSO4 0.2M, BIS-TRIS PH6.5, PEG 3350 25%, HEXAMINECOCL |
