2V3D
acid-beta-glucosidase with N-butyl-deoxynojirimycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.993, 97.210, 82.406 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 25.220 - 1.960 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3f |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.220 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 68290 | |
| <I/σ(I)> | 16.6 | 2.6 |
| Completeness [%] | 96.8 | 91.8 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M (NH4)2SO42-, 0.1 M TRIS PH 6.5, 25% (W/V) PEG3350 |
