2V36
Crystal structure of gamma-glutamyl transferase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.256, 108.766, 161.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.170 - 1.850 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dbu |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.850 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 1952022 | |
| <I/σ(I)> | 13.8 | 1.9 |
| Completeness [%] | 97.8 | 82.7 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M SODIUM HEPES PH 7.5, 25% PEG 2000K MME AND 0.2M KSCN |
