2V2P
Mutant (E53,56,57,60Q and R59M) recombinant horse spleen apoferritin cocrystallized with haemin in acidic conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.255, 181.255, 181.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 1.150 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v2l |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 88729 | |
| <I/σ(I)> | 14 | 3 |
| Completeness [%] | 98.4 | 87.8 |
| Redundancy | 6.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | RESERVOIR: CADMIUM SULFATE 0.08M, AMMONIUM SULFATE 0.5M, SODIUM ACETATE 0.1M PH 5.6, SODIUM AZIDE 0.003M. DROP: 1UL PROTEIN AND 1UL RESERVOIR |
