2V2L
Mutant (E53,56,57,60Q) recombinant horse spleen apoferritin cocrystallized with haemin in acidic conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-14 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 182.455, 182.455, 182.455 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.410 - 1.900 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v2i |
RMSD bond length | 0.019 |
RMSD bond angle | 1.556 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.490 |
Number of reflections | 21041 | |
<I/σ(I)> | 37.1 | 5.3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 10.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | RESERVOIR: CADMIUM SULFATE 0.13M, AMMONIUM SULFATE 0.8M, SODIUM ACETATE 0.1M PH 5.5, SODIUM AZIDE 0.003M. DROP: 2 UL PROTEIN AND 2 UL RESERVOIR |