2V2I
Wild type recombinant horse spleen apoferritin cocrystallized with haemin in acidic conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.334, 182.334, 182.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hrs |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 18049 | |
| <I/σ(I)> | 32.1 | 6.2 |
| Completeness [%] | 99.7 | 96.4 |
| Redundancy | 9.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | RESERVOIR: CADMIUM SULFATE 0.13M, AMMONIUM SULFATE 0.8M, SODIUM ACETATE 0.1M PH 5.5, SODIUM AZIDE 0.003M DROP 2 UL PROTEIN AND 2UL RESERVOIR |
