2V29
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT K15W)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 |
| Unit cell lengths | 82.873, 82.873, 98.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.220 - 2.000 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.040 |
| Number of reflections | 44803 | |
| <I/σ(I)> | 12.29 | 4.17 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.26 | 4.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 100 MM SODIUM ACETATE PH 4.5, 50% (V/V) ETHYLENEGLYCOL, 5% (V/V) PEG 1000 |
