2V26
Myosin VI (MD) pre-powerstroke state (Mg.ADP.VO4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.512, 93.845, 100.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.680 - 1.750 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bkh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.062 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 90108 | |
| <I/σ(I)> | 4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3-4% PEG 8000, 50MM MES PH 6.75, 100MM SAM, 1MM TCEP |
