2V24
Structure of the human SPRY domain-containing SOCS box protein SSB-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.220, 104.220, 40.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fnj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.560 |
| Number of reflections | 13208 | |
| <I/σ(I)> | 18.7 | 3.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.9 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NACACOD PH6.5, 14.4% PEG10K, 0.16 M CAAC2, 20% GLYCEROL, pH 6.50 |
