2V1Q

Atomic-resolution structure of the yeast Sla1 SH3 domain 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	46.940, 55.250, 85.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.200
R-factor	0.151
Rwork	0.148
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z9z
RMSD bond length	0.021
RMSD bond angle	1.949
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.040	0.530
Number of reflections	33200
<I/σ(I)>	19.4	2.8
Completeness [%]	100.0	100
Redundancy	5.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1