2V1B

N- and C-terminal helices of oat LOV2 (404-546) are involved in light-induced signal transduction (room temperature (293K) light structure of LOV2 (404-546))

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	293
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	35.919, 57.169, 67.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.120 - 1.550
R-factor	0.166
Rwork	0.164
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0u
RMSD bond length	0.014
RMSD bond angle	1.634
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.043	0.360
Number of reflections	19930
<I/σ(I)>	29.6	2.4
Completeness [%]	96.0	96
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1