2V15

Terbium binding in Streptococcus suis Dpr protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-19
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	104.485, 137.715, 142.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.100
R-factor	0.19
Rwork	0.188
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1umn
RMSD bond length	0.014
RMSD bond angle	1.412
Data reduction software	HKL
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000
High resolution limit [Å]	2.000	2.100
Rmerge	0.080
Number of reflections	115331
<I/σ(I)>	13.9
Completeness [%]	95.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	2UL AND 2UL VOLUME DROP, 30 % PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH, PH 7.4, HANGING DROP,16C