2V14
Kinesin 16B Phox-homology domain (KIF16B)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-03 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.979 , 0.9393 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 100.894, 100.894, 100.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.430 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.28700 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.430 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 8819 | |
| <I/σ(I)> | 29.84 | 2.17 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 27.54 | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 25% PEG 3350, 0.1 M TRIS PH 8.8, 0.2 M NH4OAC |
