2V0L

Characterization of Substrate Binding and Catalysis of the Potential Antibacterial Target N-acetylglucosamine-1-phosphate Uridyltransferase (GlmU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-01
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	108.720, 108.720, 326.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.187
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0h
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.250
Number of reflections	38714
<I/σ(I)>	27.7
Completeness [%]	99.9	99.9
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0