2V0K

Characterization of Substrate Binding and Catalysis of the Potential Antibacterial Target N-acetylglucosamine-1-phosphate Uridyltransferase (GlmU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-01
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	108.660, 108.660, 326.618
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	111.800 - 2.300
R-factor	0.19
Rwork	0.188
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.128
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.150
Number of reflections	62781
<I/σ(I)>	12.5
Completeness [%]	98.4	94.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0