2V0A
Atomic resolution crystal structure of Human Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.545, 68.220, 50.306 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 21.500 - 1.150 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.20600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.209 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 88555 | |
| <I/σ(I)> | 20 | 2.2 |
| Completeness [%] | 99.6 | 89 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.75 | 3.5 M AMMONIUM SULPHATE, 100MM NACL, 50MM ACETATE BUFFER PH 4.75 |
