2V05
CRYSTAL STRUCTURE OF CHOLINE BINDING PROTEIN F FROM STREPTOCOCCUS PNEUMONIAE. CRYSTAL FORM II.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.126, 114.936, 75.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.700 - 1.670 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.070 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 50379 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 99.1 | 96.7 |
| Redundancy | 12.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
