2V02
Recombinant vertebrate calmodulin complexed with Ba
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.410, 29.320, 53.320 |
| Unit cell angles | 89.84, 85.68, 82.80 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1up5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.090 |
| Number of reflections | 6993 | |
| <I/σ(I)> | 12.2 | 8.1 |
| Completeness [%] | 94.1 | 95.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 40-50% MPD, PH4 |
