2UZQ
Protein Phosphatase, New Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 123.925, 123.925, 174.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.770 - 2.380 |
| Rwork | 0.226 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qb0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Phasing software | MOLREP |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 58835 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 97.3 | 89.3 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PEG 8000, HEPES PH 7.0, GUANIDINIUM CHLORIDE |
