2UZC
Structure of human PDLIM5 in complex with the C-terminal peptide of human alpha-actinin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.250, 36.474, 88.706 |
| Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pkt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.380 |
| Number of reflections | 49917 | |
| <I/σ(I)> | 17.2 | 1.98 |
| Completeness [%] | 79.7 | 37 |
| Redundancy | 2.69 | 0.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350 0.20 M KSCN |
