2UYJ
Crystal structure of E. coli TdcF with bound ethylene glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.671, 86.224, 62.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.320 - 2.350 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qu9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.720 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 16863 | |
| <I/σ(I)> | 10.81 | 1.83 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG 1000, 8% PEG 8000, NO BUFFER, pH 7.00 |
