2UY8
R92A mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-11 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.486, 154.486, 122.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.120 - 2.800 |
| R-factor | 0.138 |
| Rwork | 0.134 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uw8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.612 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.040 | 2.820 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.160 | 0.350 |
| Number of reflections | 13947 | |
| <I/σ(I)> | 13.41 | 6.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.75 | 7.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |
