2UY6
Crystal structure of the P pilus rod subunit PapA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.100, 73.560, 80.480 |
| Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
| Resolution | 76.300 - 2.500 |
| R-factor | 0.2219 |
| Rwork | 0.222 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.308 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.300 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 25542 | |
| <I/σ(I)> | 6.2 | 1.9 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG8K 12%, 5% ISOPROPANOL, TRIS 0.1M PH 7.5 |
