2UXY
Aliphatic amidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 102.706, 102.706, 151.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 1.280 - 1.250 |
| R-factor | 0.1112 |
| R-free | 0.13550 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.670 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 115216 | |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 89.0 | 51.5 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | VAPOUR DIFFUSION IN SITTING DROPS OF 5 MICRO-L OF 25 MG/ML PROTEIN SOLUTION IN 50 MM TRIS-HCL PH 7.2, 5 MM DTT AND 1 MM EDTA, PLUS 2 MICRO-L OF WELL SOLUTION 15% PEG 4K AND 0.2M AMMONIUM SULPHATE PH 6.8 |
