2UXV
SufI Protein from Escherichia Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-03-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.120, 48.880, 131.960 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 59.760 - 2.610 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uxt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.13.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.150 | 0.420 |
| Number of reflections | 24830 | |
| <I/σ(I)> | 13.3 | 3.7 |
| Completeness [%] | 99.5 | 96.8 |
| Redundancy | 6.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2.35M NACL 0.1M IMIDAZOLE, PH 8.5 |
