2UX5
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 9 in the charge-separated state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-14 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.460, 139.460, 184.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.210 |
R-factor | 0.203 |
Rwork | 0.202 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.020 |
RMSD bond angle | 2.465 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.430 |
Number of reflections | 103587 | |
<I/σ(I)> | 10.29 | 3.72 |
Completeness [%] | 98.1 | 89.2 |
Redundancy | 2.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | pH 9 |