2UX2
High resolution structure of human CD59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| Unit cell lengths | 60.840, 60.840, 107.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8c |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.827 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.11.2.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.320 |
| Number of reflections | 20934 | |
| <I/σ(I)> | 29 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG8000, 0.03M POTASSIUM PHOSPHATE |
