2UX1
Identification of two zinc-binding sites in the Streptococcus suis Dpr protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.520, 137.690, 142.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 180247 | |
| <I/σ(I)> | 17.98 | 3.6 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 12.004 | 4.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | PROTEIN WAS CRYSTALLIZED FROM 30% PEG 400, 0.2 M CACL2, 0.1 M HEPES-NA, PH 7.4 AND 1 MM ZNCL2. |
