2UWR
High resolution structure of human CD59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.524, 27.902, 81.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 1.440 - 1.340 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8b |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.13.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.410 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.070 | 0.220 |
| Number of reflections | 13368 | |
| <I/σ(I)> | 16.3 | 4.5 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 5.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 23% PEG8000, 20% ETHANOL, 0.03M POTASSIUM PHOSPHATE |
