2UWM
C-TERMINAL DOMAIN(WH2-WH4) OF ELONGATION FACTOR SELB IN COMPLEX WITH SECIS RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.074, 119.843, 50.461 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.204 |
| Rwork | 0.200 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wsu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 39541 | |
| <I/σ(I)> | 14.2 | 2.1 |
| Completeness [%] | 97.3 | 79.7 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | MES PH5.5, PEG20K 18%, ETHYLENE GLYCOL 10%, NACL 0.1M, SPERMIDINE 20MM, pH 5.50 |
