2UWC
Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 6 |
| Unit cell lengths | 152.123, 152.123, 83.233 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.310 - 2.300 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1un1 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.600 |
| Number of reflections | 47776 | |
| <I/σ(I)> | 10.9 | 2.5 |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2-3 M POTASSIUM ACETATE, 2% 2, 5-METHYLPENTANE-DIOL AND 100 MM TRIS BUFFER AT PH 8.5 |
