2UWB
Crystal structure of the Nasturtium seedling mutant xyloglucanase isoform NXG1-delta-YNIIG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 6 |
| Unit cell lengths | 153.894, 153.894, 83.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uwa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.533 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 76393 | |
| <I/σ(I)> | 18.7 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.4 M SODIUM ACETATE AND 100 MM SODIUM-CACODYLATE BUFFER AT PH 6.5 |
