2UVY

Structure of PKA-PKB chimera complexed with methyl-(4-(9H-purin-6-yl)- benzyl)-amine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	72.718, 74.646, 80.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.280 - 1.950
R-factor	0.218
Rwork	0.216
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.988
Refinement software	REFMAC (5.2.0019G)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.300	1.970
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.190
Number of reflections	29879
<I/σ(I)>	6.6	3.4
Completeness [%]	97.1	87.2
Redundancy	2.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1