2UVV
Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.776, 103.725, 53.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.320 - 2.200 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2uvr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.320 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.440 |
Number of reflections | 27480 | |
<I/σ(I)> | 23.61 | 4.41 |
Completeness [%] | 99.1 | 98.6 |
Redundancy | 6.38 | 6.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.3 | 7% PEG 4000, 10 MM HEPES PH 7.3, 50 MM CA(CH3CO2)2, 50MM NACL, 3.5% GLYCEROL |