2UVO
High Resolution Crystal Structure of Wheat Germ Agglutinin in Complex with N-Acetyl-D-Glucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.390, 91.510, 94.500 |
| Unit cell angles | 90.00, 82.00, 90.00 |
Refinement procedure
| Resolution | 93.660 - 1.400 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wgt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.480 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.750 |
| Number of reflections | 137253 | |
| <I/σ(I)> | 11.87 | 2.27 |
| Completeness [%] | 93.5 | 96.3 |
| Redundancy | 3.75 | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CRYSTALLIZATION BUFFER: 20 MM SODIUM ACETATE, 6 MM CACL2, 4 % ETHANOL RESERVOIR: 0.04 M POTASSIUM DIHYDROGEN PHOSPHATE, 16 % PEG 8000, 20 % GLYCEROL |
