2UV6
Crystal Structure of a CBS domain pair from the regulatory gamma1 subunit of human AMPK in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-16 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.092, 43.775, 55.786 |
Unit cell angles | 90.00, 119.04, 90.00 |
Refinement procedure
Resolution | 34.890 - 2.000 |
R-factor | 0.176 |
Rwork | 0.172 |
R-free | 0.24700 |
Structure solution method | OTHER |
RMSD bond length | 0.013 |
RMSD bond angle | 1.316 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Refinement software | REFMAC (5.2.0019G) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.890 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.090 |
Number of reflections | 9542 |
<I/σ(I)> | 7.9 |
Completeness [%] | 97.9 |
Redundancy | 3.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8.00 |