2UV1
Hexagonal crystal form of GamS from bacteriophage lambda.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 84.479, 84.479, 105.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 105.410 - 2.600 |
| R-factor | 0.239 |
| Rwork | 0.235 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uuz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.256 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.270 |
| Number of reflections | 6851 | |
| <I/σ(I)> | 6 | 2.4 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 15.3 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES, PH 6.5, 20-25 % PEG 400, 0.1 M NACL |
