2UUU
alkyldihydroxyacetonephosphate synthase in P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2006-11-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.499, 108.913, 216.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 1.950 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uuv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.443 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.010 | 0.420 |
| Number of reflections | 177635 | |
| <I/σ(I)> | 12.5 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG4K, 200 MM LI2SO4, 100 MM TRISCL PH=8.5 |
