2UUT
The 2.4 angstrom resolution structure of the D346G mutant of the Sapporo Virus RdRp polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARREASEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.374, 93.018, 96.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.270 - 2.400 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ckw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.570 |
| High resolution limit [Å] | 2.400 | 2.420 |
| Rmerge | 0.110 | 0.220 |
| Number of reflections | 24268 | |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
