2UUS
Crystal structure of the rat FGF1-sucrose octasulfate (SOS) complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-02-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.883, 52.778, 73.127 |
| Unit cell angles | 90.00, 97.43, 90.00 |
Refinement procedure
| Resolution | 42.680 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j3p |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.518 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.680 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.170 |
| Number of reflections | 14319 | |
| <I/σ(I)> | 12.8 | 4.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 10% PEG-6000, 0.1 M CITRIC ACID PH 5.0. |
