2UUR
N-terminal NC4 domain of collagen IX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797, 0.9796, 0.8856 |
| Spacegroup name | I 4 |
| Unit cell lengths | 81.888, 81.888, 71.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.800 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 21918 | |
| <I/σ(I)> | 20.5 | 5.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES, PH 6.5 10 MM ZNSO4 20% MME-PEG 550 |
