2UUM
Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.332, 115.639, 183.258 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 182.570 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gh0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.378 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.020 |
| Number of reflections | 86909 | |
| <I/σ(I)> | 9.19 | 3.9 |
| Completeness [%] | 96.6 | 93.8 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.01M SODIUM PHOSPHATE PH 6.5, 0.72 M SOD. FORMATE, AND 13.5% PEG 4K |
