2UUD
Crystal structure of the TEPC15-Vk45.1 anti-2-phenyl-5-oxazolone NQ10- 1.12 scFv in complex with the hapten
Replaces: 2CJVExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-13 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.944, 75.657, 52.854 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.640 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 11988 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 0.1 M TRIS-HCL, PH 7.6, 0.2 M AMMONIUM SULPHATE, 30% PEG 4K |
