2UP1

STRUCTURE OF UP1-TELOMERIC DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Detector	BRANDEIS
Spacegroup name	P 43 21 2
Unit cell lengths	51.200, 51.200, 171.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 2.100
R-factor	0.195
Rwork	0.195
R-free	0.24900
Structure solution method	MIRAS
Starting model (for MR)	1up1
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.051	0.218
Number of reflections	14247
<I/σ(I)>	14.2	10
Completeness [%]	96.1	86.5
Redundancy	3.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	glycerol	15 (%)
4	1	reservoir		2.0 (M)