Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-08 |
| Detector | SIEMENS |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.500, 106.500, 49.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.930 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AAK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.400 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.110 | |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.154 * | |
| Total number of observations | 25491 * | |
| Number of reflections | 6557 | 720 * |
| <I/σ(I)> | 8 | 1 |
| Completeness [%] | 93.0 | 62 |
| Redundancy | 3.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | 23 * | PROTEIN WAS CRYSTALLIZED FROM 0.6 M SODIUM CITRATE, 100 MM HEPES, PH 7.4, USING HANGING DROP TECHNIQUE AT 23 DEG, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 0.3 (M) | |
| 3 | 1 | drop | HEPES | 0.05 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.6 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) |
