2TYS

CRYSTAL STRUCTURES OF MUTANT (BETAK87T) TRYPTOPHAN SYNTHASE ALPHA2 BETA2 COMPLEX WITH LIGANDS BOUND TO THE ACTIVE SITES OF THE ALPHA AND BETA SUBUNITS REVEAL LIGAND-INDUCED CONFORMATIONAL CHANGES

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1992-12
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	185.800, 62.300, 67.800
Unit cell angles	90.00, 94.10, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.171 *
Rwork	0.171
R-free	0.21900 *
Structure solution method	ISOMORPHOUS WITH PDB ENTRY 1WSY.
RMSD bond length	0.009
RMSD bond angle	0.018
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.044
Number of reflections	50490
<I/σ(I)>	18.3	3.7
Completeness [%]	84.6	46.8
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.8		pH is adjusted to 7.8 with NaOH *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Bicine	50 (mM)
2	1	1	Na-EDTA	1 (mM)
3	1	1	spermine	0.8-1.5 (mM)
4	1	1	PEG8000	12 (%)