Experimental procedure
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.320, 68.320, 181.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.300 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 * |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.065 * |
| Number of reflections | 8505 * |
| Completeness [%] | 94.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | H. E. Parge, (1984) J. Mol. Biol., 180, 1189. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (%(w/v)) | |
| 10 | 1 | reservoir | beta-mercaptoethanol | 7 (mM) | |
| 11 | 1 | reservoir | EDTA | 1 (mM) | |
| 12 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 7 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | 0.02 (%(w/v)) | ||
| 7 | 1 | reservoir | disammonium hydrogen phosphate | 1.3-1.5 (M) | |
| 8 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 9 | 1 | reservoir | 50 (mM) |
