2TOH

TYROSINE HYDROXYLASE CATALYTIC AND TETRAMERIZATION DOMAINS FROM RAT

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01
Detector	MARRESEARCH
Spacegroup name	F 2 2 2
Unit cell lengths	189.464, 148.595, 56.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.207
Rwork	0.207
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1-H
RMSD bond length	0.020
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.065	0.310
Number of reflections	17449
<I/σ(I)>	16	4.5
Completeness [%]	95.1	81.6
Redundancy	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.9	4 *	pH 7.9

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	16 (mg/ml)
2	1	2	ammonium sulfate	1.15 (M)
3	1	2	PEG200	3.2 (%)
4	1	2	glycerol	5 (%)
5	1	2	Tris	100 (mM)
6	1	2	7,8-dihydrobiopterin	3 (mM)