2TMD

CORRELATION OF X-RAY DEDUCED AND EXPERIMENTAL AMINO ACID SEQUENCES OF TRIMETHYLAMINE DEHYDROGENASE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	147.750, 71.950, 83.820
Unit cell angles	90.00, 97.69, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.154
Rwork	0.154
RMSD bond length	0.008
RMSD bond angle	1.281
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.032 *
Total number of observations	430000 *
Number of reflections	133000 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		taken from Lim, L. et al (1982). J. Mol. Biol., 162, 869-876., macro-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10-12 (%)